COT I Human DNA
- Product Code: Bioron
Cat No:
401001
Descriptions
- COT I Human DNA is prepared exclusively from male human placental DNA by shearing, denaturing, and reannealing under conditions that enrich repetitive elements. The COT I fraction of human genomic DNA consists largely of rapidly annealing repetitive elements.
- Quantity: 500µg
- Quality Control: Avarage fragments size - 50-300 bp;A260/A280: 1.78;amount of genomic (non-repetitive DNA) - 2%.The sample as well as original material were tested for the absence of HIV1,2 RNA, HCV RNA, HBV DNA.
Concentration: >1 mg/ml
Applications
COT I Human DNA can be used for suppressing crosshybridization to human repetitive DNA in filter and microarray hybridizations and in situ hybridization experiments. Repetitive elements present in a probe may result in unspecific hybridization signals. To enable specific hybridization of the probe to the chromosomal target site the probe is denatured together with an excess of unlabeled COT I Human DNA as a competitor. As a result single-cope gene hybridization is not hindered by repetitive sequences.